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Seafood & Fisheries Articles
Bioactive Compounds From Algae And Bacteria In Marine Environment
The discovery of new bioactive compounds necessarily involves previously diversity studies, because by knowing the type of microorganisms that reside in a certain environment, it is possible to design cultivation techniques adapted for all the microbial communities present in a certain ambience. Read the full article here.
Quorum sensing: How Bacteria Talk to Each Other
Quorum sensing (QS) is a cell-to-cell signaling mechanism that refers to the ability of bacteria to respond to chemical hormone-like molecules called autoinducers. When an autoinducer reaches a critical threshold, the bacteria detect and respond to this signal by altering their gene expression. Quorum sensing thus enables bacteria to co-ordinate and respond quickly to environmental changes, such as the availability of nutrients, other microbes or toxins in their environment. Read the full article here.
Cell Culture & Its Application
Cell culture is the process by which prokaryotic, eukaryotic or plant cells are grown under controlled conditions. But in practice it refers to the culturing of cells derived from animal cells.Cell culture was first successfully undertaken by Ross Harrison in 1907. Roux in 1885 for the first time maintained embryonic chick cells in a cell culture. The cells may be removed from the tissue directly and disaggregated by enzymatic or mechanical means before cultivation, or they may be derived from a cell line or cell strain that has already been already established. Read the full article here.
ELISA - Emerging molecular tool in fish disease diagnosis
An antibody (Ab) reacts with the concerned antigen (Ag) in a highly specific manner (i.e. an antibody reacts only with that determinant or region of an antigen for which it is specific) to produce an Antigen-Antibody (Ag-Ab) complex. The antigen-antibody interaction in a bimolecular association similar to an enzyme-substrate interaction, with an important distinction: it does not lead to an irreversible chemical alteration in either the antibody or the antigen. The association between an antibody and an antigen involves various non-covalent interactions between the antigenic determinant, or epitope, of the antigen and the variable-region (VH/VL) domain of the antibody molecule, particularly the hyper variable regions, or complementarily-determining regions (CDRs). Read the full article here.
Importance of Polymerase Chain Reaction (PCR) in Fish Disease Diagnosis
The polymerase chain reaction (PCR) is a technique widely used in molecular biology. It derives its name from one of its key components, a DNA polymerase used to amplify a piece of DNA by in vitro enzymatic replication. As PCR progresses, the DNA generated is used as a template for replication. This sets in motion a chain reaction in which the DNA template is exponentially amplified. With PCR it is possible to amplify a single or few copies of a piece of DNA across several orders of magnitude, generating millions or more copies of the DNA piece. Read the full article here.
Anaesthesia And Anaesthetics For The Controlled Management Of Fresh Water Fishes
Anesthesia is a state caused by an applied external agent resulting in a loss of sensation through depression of the nervous system. The use of anesthetics in fisheries and aquaculture research greatly facilitates in procedures including induction of breeding, handling during stripping and transport of broodstock. Read the full article here.
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